Abstract
We propose and analyze the use of fluorescence phase-shifting interferometric (PSI) imaging for single particle tracking along 1 μm-depth (z) trajectories. While spatial PSI using a single camera attains a maximal twofold improvement in the z-localization precision compared to temporal PSI for <0.1 μm2/s diffusion, temporal PSI shows a fourfold larger field-of-view.
© 2013 Optical Society of America
PDF Article | Presentation VideoMore Like This
Ying-Hsiu Lin, Hsiao-Mei Wu, and Chia-Lung Hsieh
NM3C.4 Novel Techniques in Microscopy (NTM) 2015
Tatiana Latychevskaia, Jessica Britschgi, and Hans-Werner Fink
DW1A.3 Digital Holography and Three-Dimensional Imaging (DH) 2013
David I. Serrano-Garcia, Yukitoshi Otani, Amalia Martinez-Garcia, and Noel-Ivan Toto-Arellano
Tu5 CIOMP-OSA Summer Session on Optical Engineering, Design and Manufacturing (SumSession) 2013