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Conferences > CLEO_SI > 2014 > STh5C > STh5C.2

In Vivo Three-photon Calcium Imaging of Brain Activity from Layer 6 Neurons in Mouse Brain

Dimitre G. Ouzounov, Nicholas Horton, Tianyu Wang, Danielle Feng, Nozomi Nishimura, and Chris Xu

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Abstract

We demonstrate three-photon microscopy (3PM) at 1300-nm excitation for imaging neuronal activity as deep as layer-6 (L6) of adult mouse cortex using genetically-encoded calcium indicators. In vivo imaging of subcortical, GFP-labeled neurons is also demonstrated.

© 2014 Optical Society of America

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