Doubling the Sensitivity of Multiphoton Frequency-Domain Fluorescence Lifetime Images

Yide Zhang; Genevieve D. Vigil; Aamir A. Khan; Scott S. Howard

doi:10.1364/CLEO_SI.2017.SM3C.6

Conference on Lasers and Electro-Optics
OSA Technical Digest (online) (Optica Publishing Group, 2017),
paper SM3C.6
•https://doi.org/10.1364/CLEO_SI.2017.SM3C.6

Doubling the Sensitivity of Multiphoton Frequency-Domain Fluorescence Lifetime Images

Yide Zhang, Genevieve D. Vigil, Aamir A. Khan, and Scott S. Howard

CLEO: Science and Innovations 2017

San Jose, California United States
14–19 May 2017
ISBN: 978-1-943580-27-9

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In Vivo/Deep Tissue Imaging (SM3C)

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Y. Zhang, G. D. Vigil, A. A. Khan, and S. S. Howard, "Doubling the Sensitivity of Multiphoton Frequency-Domain Fluorescence Lifetime Images," in Conference on Lasers and Electro-Optics, OSA Technical Digest (online) (Optica Publishing Group, 2017), paper SM3C.6.

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Abstract

We demonstrate super-sensitive multiphoton frequency-domain fluorescence lifetime images using the recently developed DC&1ω method by showing a two-fold improvement in imaging sensitivity compared to the conventional 1ω phase fluorometry.

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