Dual-band 2-photon excitation dual-region imaging with subcellular resolution across 25mm2 field of view

Che-Hang Yu; Jeffery Stirman; Spencer L. Smith

doi:10.1364/CLEO_SI.2021.SW2D.1

Conference on Lasers and Electro-Optics
OSA Technical Digest (Optica Publishing Group, 2021),
paper SW2D.1
•https://doi.org/10.1364/CLEO_SI.2021.SW2D.1

Dual-band 2-photon excitation dual-region imaging with subcellular resolution across 25mm² field of view

Che-Hang Yu, Jeffery Stirman, and Spencer L. Smith

CLEO: Science and Innovations 2021

San Jose, California United States
9–14 May 2021
ISBN: 978-1-943580-91-0

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C. Yu, J. Stirman, and S. L. Smith, "Dual-band 2-photon excitation dual-region imaging with subcellular resolution across 25mm2 field of view," in Conference on Lasers and Electro-Optics, J. Kang, S. Tomasulo, I. Ilev, D. Müller, N. Litchinitser, S. Polyakov, V. Podolskiy, J. Nunn, C. Dorrer, T. Fortier, Q. Gan, and C. Saraceno, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper SW2D.1.

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Abstract

We developed a dual-wavelength excitation, large field-of-view (25 mm²) two-photon microscope with temporal multiplexing and two completely independent scan engines coupled into the same objective for imaging in multiple areas with multiple wavelengths simultaneously.

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