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Conferences > CLEO_SI > 2023 > STu4F > STu4F.5

Label-Free Three-Photon Deep Imaging with High Power Femtosecond Yb-fiber Laser

Alma Fernández, Anton Classen, Nitya Kalyani Josyula, Paul Straight, and Aart Verhoef

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Abstract

We demonstrate simultaneous multicolor two- and three-photon imaging of bacterial filaments using a single high-power femtosecond Yb-fiber laser. Our experiments demonstrate the superior signal-to-background ratio obtainable with three-photon imaging deep below the sample surface.

© 2023 The Author(s)

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