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Partially coherent phase microscopy with arbitrary illumination source shape

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We propose a method that recovers phase from a stack of through-focus intensity images taken with a partially coherent microscope employing illumination of any arbitrary source shape. Our algorithm uses a Kalman filtering approach, which is fast, accurate and robust to noise. We validate the method in simulation and with experimental data taken on a commercial microscope with varying condenser apertures in Köhler geometry.

© 2014 Optical Society of America

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