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Conferences > COSI > 2016 > CW2D > CW2D.3

Experimental Implementation of Wavefront Encoding in 3D Widefield Fluorescence Microscopy Using a Fabricated Phase Mask Designed to Reduce System Depth Variability

Nurmohammed Patwary, Sharon V. King, Hasti Shabani, and Chrysanthe Preza

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Abstract

Wavefront encoded (WFE) three dimensional fluorescent imaging is studied with a fabricated squared cubic (SQUBIC) phase mask. Experimental results demonstrate that, the WFE system can produce results predicted by simulations, and intermediate WFE images show 3% variability over a 60 µm imaging depth.

© 2016 Optical Society of America

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