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Double-Cubic Point Spread Function for 3D Extended-Depth Localization Microscopy

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Abstract

We report the design and implementation of a new pupil-engineered phase function that enables simple and robust 3D localization microscopy with a ten-fold extension in depth-of-field. Applications include single-particle tracking, super resolution microscopy and lab-on-chip. We demonstrate its application to in vivo mapping of blood flow in zebrafish.

© 2018 The Author(s)

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