Quantification of Metabolic Function in the Retina Using Spectral Imaging and Phasor-FLIM

Daniel Geddes; Julia Noorbakhsh; Zhiyuan Yang; Miguel Preciado; Margaret Normand; Kenneth Smith; Andrew R. Harvey

doi:10.1364/COSI.2022.JW4C.3

Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP)
Technical Digest Series (Optica Publishing Group, 2022),
paper JW4C.3
•https://doi.org/10.1364/COSI.2022.JW4C.3

Quantification of Metabolic Function in the Retina Using Spectral Imaging and Phasor-FLIM

Daniel Geddes, Julia Noorbakhsh, Zhiyuan Yang, Miguel Preciado, Margaret Normand, Kenneth Smith, and Andrew R. Harvey

Computational Optical Sensing and Imaging 2022

Vancouver, British Columbia Canada
11–15 July 2022
ISBN: 978-1-957171-09-8

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Advances in the Design, Fabrication and Characterization of Optical Sensors I (Joint COSI and IS) (JW4C)

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D. Geddes, J. Noorbakhsh, Z. Yang, M. Preciado, M. Normand, K. Smith, and A. R. Harvey, "Quantification of Metabolic Function in the Retina Using Spectral Imaging and Phasor-FLIM," in Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP), Technical Digest Series (Optica Publishing Group, 2022), paper JW4C.3.

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Abstract

High clutter and low signal mean that existing retinal-imaging techniques are not well suited to measuring retinal metabolism. We describe spectral/lifetime phasor measurement of fluorescent biomarkers as a route to routine assessment of retinal health.

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