Quantitative Phase Imagery with Total Internal Reflection Holographic Microscopy

William M. Ash; Myung K. Kim

doi:10.1364/DH.2009.DTuA4

Advances in Imaging
OSA Technical Digest (CD) (Optica Publishing Group, 2009),
paper DTuA4
•https://doi.org/10.1364/DH.2009.DTuA4

Quantitative Phase Imagery with Total Internal Reflection Holographic Microscopy

William M. Ash and Myung K. Kim

Digital Holography and Three-Dimensional Imaging 2009

Vancouver Canada
26–30 April 2009
ISBN: 978-1-55752-871-1

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Holographic Microscopy (DTuA)

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W. M. Ash and M. K. Kim, "Quantitative Phase Imagery with Total Internal Reflection Holographic Microscopy," in Advances in Imaging, OSA Technical Digest (CD) (Optica Publishing Group, 2009), paper DTuA4.

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Abstract

Total internal reflection holographic microscopy is used to produce quantitative phase images of biological specimens. Quantitative cellular images, algorithmic considerations and optomechanical implementation are presented and discussed. Applications include characterization of membranes, motility and metastasis.

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