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Conferences > DH > 2012 > DSu5B > DSu5B.5

High fidelity reconstruction of three-dimensional objects by FINCH fluorescence microscopy

Nisan Siegel, Joseph Rosen, and Gary Brooker

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Abstract

FINCH holography produces super-resolved images above and below the focal plane of the microscope objective but with different magnifications. A method is presented to faithfully reconstruct 3D objects with dimensional fidelity.

© 2012 Optical Society of America

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