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Speckle-based volume holographic microscopy for optically sectioned multi-depths fluorescent imaging

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The optically sectioned imaging techniques can provide good interpretation for fine and detailed features of volumetric biological sample. However, the existing optical sectioned systems need the lateral or axial dimensional scanning for volumetric object. We report a speckle-based volume holographic microscopy to generate non-scanning, multi-depths images with good optically sectioned capability, validated with fluorescent 25 μm beads and mice intestine experiments.

© 2015 Optical Society of America

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