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Conferences > DH > 2016 > DT4G > DT4G.2

Efficient holographic multi-site two-photon fluorescence for functional calcium imaging of neuronal circuits

Michael Lawrence Castanares, Vini Gautam, Hans Bachor, and Vincent R. Daria

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Abstract

We present a technique to improve the two-photon fluorescence yield from holographically projected multiple foci with less photo-damage on living cells. By positioning multiple foci to neurons in a circuit, we observed correlated calcium activity.

© 2016 Optical Society of America

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