Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Computational refocusing in phase-resolved confocal microscopy

Not Accessible

Your library or personal account may give you access

Abstract

We demonstrate numerical refocusing in monochromatic, phase-resolved confocal microscopy. The refocusing method is based on the pseudoinverse of the forward operator of the confocal imaging system and a subsequent re-imaging of the object to a new focal plane. The application of the model requires the acquisition of amplitude- and phase-resolved confocal data. To this end, we implement synthetic optical holography, a recently introduced holographic modality for quantitative phase imaging in scanning optical microscopy, in a homebuilt confocal setup. Using a resolution test target, we demonstrate that numerical refocusing restores a spatial resolution near the theoretical diffraction limit for situations where the object is located up to 60 microns away from the system’s focal plane. Our method brings numerical refocusing to monochromatic confocal microscopy, allowing for refocusing out-of-focus images after image acquisition.

© 2016 Optical Society of America

PDF Article
More Like This
Synthetic holographic phase imaging in confocal microscopy and applications

M. Schnell, S. Buercklin, P. Sarriugarte, M. J. Perez-Roldan, R. Hillenbrand, and P. S. Carney
FW2C.4 Frontiers in Optics (FiO) 2016

Dynamic surface profiling with synthetic holographic confocal microscopy

M. Schnell, P. S. Carney, and R. Hillenbrand
JTh2A.78 Frontiers in Optics (FiO) 2016

Fast Refocusing Based on the Phase in the Fourier Domain in Multispectral Digital Holographic Microscopy

Jérôme Dohet-Eraly, Catherine Yourassowsky, and Frank Dubois
DTh1E.4 Digital Holography and Three-Dimensional Imaging (DH) 2016

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved