Optical Diffraction Tomography (ODT) for Label-Free Imaging of Large 3D Biological Samples

Elizabeth E. Antoine; Joowon Lim; Ahmed B. Ayoub; Nathalie Brandenberg; Demetri Psaltis

doi:10.1364/DH.2020.HF1G.2

Imaging and Applied Optics Congress
OSA Technical Digest (Optica Publishing Group, 2020),
paper HF1G.2
•https://doi.org/10.1364/DH.2020.HF1G.2

Optical Diffraction Tomography (ODT) for Label-Free Imaging of Large 3D Biological Samples

Elizabeth E. Antoine, Joowon Lim, Ahmed B. Ayoub, Nathalie Brandenberg, and Demetri Psaltis

Digital Holography and Three-Dimensional Imaging 2020

Washington, DC United States
22–26 June 2020
ISBN: 978-1-943580-77-4

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Tomographic Methods and Imaging (HF1G)

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E. E. Antoine, J. Lim, A. B. Ayoub, N. Brandenberg, and D. Psaltis, "Optical Diffraction Tomography (ODT) for Label-Free Imaging of Large 3D Biological Samples," in Imaging and Applied Optics Congress, OSA Technical Digest (Optica Publishing Group, 2020), paper HF1G.2.

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Abstract

We developed a new ODT microscope for large-sample imaging which incorporates a custom sample holder and two complementary scanning modalities. We measured for the first time the 3D refractive index of an unlabeled unmodified stem-cell-derived organoid.

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