Phase distortion in holographic scanning microscopy: source and elimination

M.S. Muravyeva; M.S. Muravyeva; I.V. Mukhina; I.V. Mukhina; Yu.N. Zakharov

doi:10.1364/3D.2020.JTh2A.27

Imaging and Applied Optics Congress
OSA Technical Digest (Optica Publishing Group, 2020),
paper JTh2A.27
•https://doi.org/10.1364/3D.2020.JTh2A.27

Phase distortion in holographic scanning microscopy: source and elimination

M.S. Muravyeva, I.V. Mukhina, and Yu.N. Zakharov

Digital Holography and Three-Dimensional Imaging 2020

Washington, DC United States
22–26 June 2020
ISBN: 978-1-943580-77-4

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Joint Poster Session III (JTh2A)

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M. S. Muravyeva, I. V. Mukhina, and Y. N. Zakharov, "Phase distortion in holographic scanning microscopy: source and elimination," in Imaging and Applied Optics Congress, OSA Technical Digest (Optica Publishing Group, 2020), paper JTh2A.27.

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Abstract

The ZEISS LSM-510 laser scanning microscope was modified; as a result, a system for recording digital holograms in the scanning mode of the microscope was obtained. The features of the reconstruction of recorded holograms are considered both theoretically and by simulation. Phase distortion impact is investigated. Algorithm and MatLab code for reconstruction of holograms was created allowing correct restoration both intensity and phase of the object wave. Comments and questions should be directed to the OSA Conference Papers staff (tel: +1 202.416.6191, e-mail: cstech@osa.org).

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