4D object tracking in lensless digital in-line holographic microscopy aided by dark-field based autofocusing metric

Maciej Trusiak; Mikołaj Rogalski; Julianna Winnik; Jose Angel Picazo-Bueno; Vicente Micó

doi:10.1364/DH.2021.DW4C.5

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP)
OSA Technical Digest (Optica Publishing Group, 2021),
paper DW4C.5
•https://doi.org/10.1364/DH.2021.DW4C.5

4D object tracking in lensless digital in-line holographic microscopy aided by dark-field based autofocusing metric

Maciej Trusiak, Mikołaj Rogalski, Julianna Winnik, Jose Angel Picazo-Bueno, and Vicente Micó

Digital Holography and Three-Dimensional Imaging 2021

Washington, DC United States
19–23 July 2021
ISBN: 978-1-943580-89-7

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Digital Holographic Microscopy II (DW4C)

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M. Trusiak, M. Rogalski, J. Winnik, J. A. Picazo-Bueno, and V. Micó, "4D object tracking in lensless digital in-line holographic microscopy aided by dark-field based autofocusing metric," in OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP), H. Hua, B. Javidi, M. Martinez-Corral, O. Matoba, A. Stern, S. Thibault, T. Alieva, J. Ke, F. Willomitzer, F. Okten, P. Silveira, P. Banerjee, E. Stoykova, D. Chu, J. Park, F. Imai, C. Joo, M. Digman, D. Gardner, S. Gladysz, D. LeMaster, S. Basu, and O. Korotkova, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper DW4C.5.

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Abstract

Volumetric tracking of dynamic objects is vital in biomedical and technical sciences. Lensless digital in-line holographic microscopy offers unique setup compactness and high analysis throughput. We propose new automatic tracking algorithm driven by computational d ark-field based autofocusing metric. Method is validated in live sperm cell study.

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