Multifocal Confocal Fluorescence Microscopy Using Volume Holographic Array Illumination

Surag Athippillil Suresh; Sunil Vyas; J. Andrew Yeh; Yuan Luo; Yuan Luo; Yuan Luo

doi:10.1364/DH.2022.M5A.6

Digital Holography and 3-D Imaging 2022
Technical Digest Series (Optica Publishing Group, 2022),
paper M5A.6
•https://doi.org/10.1364/DH.2022.M5A.6

Multifocal Confocal Fluorescence Microscopy Using Volume Holographic Array Illumination

Surag Athippillil Suresh, Sunil Vyas, J. Andrew Yeh, and Yuan Luo

Digital Holography and Three-Dimensional Imaging 2022

Cambridge United Kingdom
1–4 August 2022
ISBN: 978-1-957171-12-8

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S. A. Suresh, S. Vyas, J. A. Yeh, and Y. Luo, "Multifocal Confocal Fluorescence Microscopy Using Volume Holographic Array Illumination," in Digital Holography and 3-D Imaging 2022, D. Chu, J. Park, C. Cheng, and P. Ferraro, eds., Technical Digest Series (Optica Publishing Group, 2022), paper M5A.6.

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Abstract

A multifocal confocal fluorescence microscopy system is designed and developed by incorporating photopolymer (PQ-PMMA) based volume holographic optical element in the illumination path for generating multifocal super Gaussian (SG) beam spots to reduce the scanning time.

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