What Can Digital Processing Do for 3-D Super-Resolution Microscopy?

Rafael Piestun

doi:10.1364/DIPA.2010.DTuA1

Imaging and Applied Optics Congress
OSA Technical Digest (CD) (Optica Publishing Group, 2010),
paper DTuA1
•https://doi.org/10.1364/DIPA.2010.DTuA1

What Can Digital Processing Do for 3-D Super-Resolution Microscopy?

Rafael Piestun

Digital Image Processing and Analysis 2010

Tucson, Arizona United States
7–8 June 2010
ISBN: 978-1-55752-892-6

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Medical Image Processing I (DTuA)

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R. Piestun, "What Can Digital Processing Do for 3-D Super-Resolution Microscopy?," in Imaging and Applied Optics Congress, OSA Technical Digest (CD) (Optica Publishing Group, 2010), paper DTuA1.

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Abstract

Novel three-dimensional fluorescence microscopy techniques enable diffraction unlimited imaging. Resolution is limited by the photon count and the underlying noise. I discuss the role digital design and post-processing plays in attaining the new fundamental limits.

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