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3D Reconstruction of Fluorescence Microscopy Image Intensities Using Multiple Depth-Variant Point-Spread Functions

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Abstract

We show that the use of multiple depth-variant point-spread functions in 3D fluorescence intensity reconstruction provides improved optical sectioning over deconvolution methods by correcting depth-induced aberrations in 3D cell images. ©2010 Optical Society of America

© 2010 Optical Society of America

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