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Wavelength agile fluorescence microscopy filter using photorefractive barium titanate

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Abstract

We describe a wavelength-agile holographic notch filter (WAHNF) suitable for attenuating scattered laser light from the fluorescence signal in laser induced fluorescence (LIF) applications. The WAHNF utilizes a photorefractive material, specifically BaTiO3, and the technique of two beam coupling to attenuate the coherent portion of the signal to be detected1. Static holograms recorded in film emulsions are routinely used for this purpose but have the limitation that they are fixed notch-band devices. In contrast, the WAHNF enables tuning or switching the excitation wavelength while passively adapting to maintain the notch band centered on the laser wavelength.

© 1996 Optical Society of America

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