Abstract

The vast majority information about cells and cell organellae structure were obtained by means of transmission electron microscopy investigation of cells serial thin sections. However often it is very difficult to derive information about 3-D structure of specimens from such electron micrographs. A new program restoring 3-D image of cells from the serial thin sections micrographs have been developed in our lab. The program makes it possible to visualize a 3-D image of cell and obtain image of inner cell structure in arbitrary plane. The plague bacteria and anthrax cells with spore were visualized with resolution about 70 nm by means of the program.

© 2001 OSA/SPIE