Combined effect of multiple light scattering and radiative transport on fluorescence emission

Sandrina P. Barbosa; Aleksandre Fedorov; Eduardo N. Pereira; José M.G. Martinho; Mário N. Berberan-Santos

doi:10.1364/ECBO.2003.5138_310

Photon Migration and Diffuse-Light Imaging
Proc. SPIE (Optica Publishing Group, 2003),
paper 5138_310
•https://doi.org/10.1364/ECBO.2003.5138_310

Combined effect of multiple light scattering and radiative transport on fluorescence emission

Sandrina P. Barbosa, Aleksandre Fedorov, Eduardo N. Pereira, José M.G. Martinho, and Mário N. Berberan-Santos

European Conference on Biomedical Optics 2003

Munich Germany
ISBN: 0-8194-5008-1

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S. P. Barbosa, A. Fedorov, E. N. Pereira, J. M. G. Martinho, and M. N. Berberan-Santos, "Combined effect of multiple light scattering and radiative transport on fluorescence emission," in Photon Migration and Diffuse-Light Imaging, D. Boas, ed., Vol. 5138 of Proc. SPIE (Optica Publishing Group, 2003), paper 5138_310.

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Abstract

The combined effect of multiple light scattering and radiative transport on fluorescence decays is experimentally studied. The experimental setup consists of a 15 cm cubic cell containing a highly fluorescent dye solution and scatterers (either silica particles or milk). The effect of fluorophore concentration was studied, in the absence and presence of scatterer. The effect of excitation and detection geometry was also studied. It is concluded that light scattering can have a significant effect on the fluorescence decays, namely on the mean decay times, and the results can be explained on the basis of the spatial distributions of the successive generations of excited molecules.

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