Abstract
The new technique of biological medium structure estimation based on optical diffraction filtration method is represented. Biological medium is considered as a superposition of cluster molecular structures. Depending on wavelength of probing optical radiation, during scattering the different particles participate which make a unique volume diffraction grating for each wavelength. The probing radiation characteristics variation allow to estimate the structure of the researched medium. Experiments on studying of coherent and not coherent optical radiation with biological media interaction have been carried out. The images of rabbits tissues, human tissues in vivo and fluoroplastic images were processed. For the first time scattered radiation image processing technique based on signal decomposition of the Hermite functions which are the eigenfunctions of Fourier transform was applied for such class of tasks. It has allowed to reveal in strongly scattered radiation a structural component. Estimating the characteristics of a structural component it is possible to receive geometrical parameters appropriate to probing radiation diffraction grating. The offered technique is perspective for studying biological molecular structures and creating tomographs of a new class.
© 2003 SPIE
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