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Multi-photon imaging using a Ti: sapphire regenerative amplifier

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Abstract

It is shown that two-photon fluorescence images can be obtained almost throughout the entire grey-matter layers of the mouse neocortex by using optically amplified femtosecond pulses. The maximum imaging depth is not limited by the available excitation power but instead by the generation of out-of focus fluorescence.

© 2003 SPIE

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