Abstract
Two photon microscopy is a powerful tool for cells or tissues imaging. However it presents the drawback of being a laser-scanning technique leading to long time acquisition for 3D images. To preserve biological samples from too long experiments and provide a more complete spectroscopic tool we developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while keeping the possibility to measure both intensity and lifetime images for all multifocal points.
© 2003 SPIE
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