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Adaptive control of two-photon fluorescence from green fluorescent protein by shaped femtosecond excitation pulses

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Abstract

Two-photon excited fluorescence process of green fluorescent protein was adaptively controlled by shaped fem-tosecond excitation laser pulses. The phase control was given by a fused-silica spatial light modulator. We could completely intentionally increase and decrease of fluorescence efficiency against the excitation intensity. This fully-controllable method can become a robust solution for various practical problems such as photobleaching of the samples on a two-photon fluorescence microscopy.

© 2003 SPIE

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