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Sensing Cellular Function and Molecular Activity in vivo using Fluorescence Lifetime Imaging Microscopy (FLIM)

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Abstract

We present design features and applications of a novel UV-visible-NIR FLIM system with optical sectioning, high resolution (50ps), and large temporal dynamic range (750ps–1µs) to probe cellular metabolism and detect molecular interactions in vivo.

© 2005 Optical Society of America

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