Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Fluorescence reference materials used for optical and biophotonic applications

Not Accessible

Your library or personal account may give you access

Abstract

Fluorescence techniques are known for their high sensitivity and are widely used as analytical tools and detection methods for product and process control, material sciences, environmental and bio-technical analysis, molecular genetics, cell biology, medical diagnostics, and drug screening.

According to DIN/ISO 17025 certified standards are used for fluorescence diagnostics having the drawback of giving relative values for fluorescence intensities only.

Therefore reference materials for a quantitative characterization have to be related directly to the materials under investigation. In order to evaluate these figures it is necessary to calculate absolute numbers like absorption/excitation cross sections and quantum yield. This can be done for different types of dopands in different materials like glass, glass ceramics, crystals or nano crystalline material embedded in polymer matrices.

Based on the optical spectroscopy data we will discuss options for characteristic doped glasses and glass ceramics with respect to scattering and absorption regime. It has shown recently for YAG:Ce glass ceramics that for a proper determination of the quantum efficiency in these highly scattering media a reference material with similar scattering and fluorescent properties is required.

This may be performed using the emission decay measurement diagnostics, where the decay time is below 100 ns.

In this paper we present first results of these aspects using well performing LUMOGEN RED organic pigments for a comparison of mainly transparent glass with glass ceramics doped with various amounts of dopands e.g. ions of raw earth elements and transition metals. The LUMOGEN red is embedded in silica and polyurethane matrices.

Characterisations on wavelength accuracy and lifetime for different environmental conditions (temperature, UV irradiation) have been performed. Moreover intensity patterns and results for homogeneity, isotropy, photo and thermal stability will be discussed. In a next step we will show the transfer of the characterisation methods to inorganic fluophores (YAG:Ce) in silicon.

Fluorescence (steady state, decay time) and absorption (remission, absorption) spectroscopy working in different temperature regimes (10 – 350 K) are employed diagnostic methods in order to get a microscopic view of the relevant physical processes and to prove the correctness of the obtained data.

The work is funded by BMBF under project number 13N8849.

© 2007 SPIE

PDF Article
More Like This
Radiation Defects in Nonlinear Optical Materials Used for Space-Based Applications

Galina Malovichko, Martin Meyer, and Valentin Grachev
SThB4 Frontiers in Optics (FiO) 2007

Optical properties of green fluorescent proteins and their applications on virus infection

Ja-Yun Lee, Chia-Yun Kao, Ying-Ju Chen, Tzong-Yuan Wu, and I-Jen Hsu
6626_5 European Conference on Biomedical Optics (ECBO) 2007

Applications of Mixture Oxide Materials for fs Optics

M. Jupe, M. Lappschies, L. Jensen, K. Starke, and D. Ristau
TuA6 Optical Interference Coatings (OIC) 2007

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved