Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Analysis of tissue specific progenitor cell differentiation using FT-IR

Not Accessible

Your library or personal account may give you access


Tissue specific progenitor cells and its differentiations have got a lot of attentions in regenerative medicine. The process of differentiations, the formation of tissues, has become better understood by the study using a lot of cell types progressively. These studies of cells and tissue dynamics at molecular levels are carried out through various approaches like histochemical methods, application of molecular biology and immunology. However, in case of using regenerative sources (cells, tissues and biomaterials etc.) clinically, they are measured and quality-controlled by non-contact and non-destructive methods from the view point of safety. Or the analysis with small quantities of materials could be possible if the quantities of materials are acceptable. A non-contact and non-destructive quality control method has been required. Recently, the use of Fourier Transform Infrared spectroscopy (FT-IR) has been used to monitor biochemical changes in cells, and has gained considerable importance. The changes in the cells and tissues, which are subtle and often not obvious in the histpathological studies, are shown to be well resolved using FT-IR. Moreover, although most techniques designed to detect one or a few changes, FT-IR is possible to identify the changes in the levels of various cellular biochemicals simultaneously under in vivo and in vitro conditions. The objective of this study is to establish the infrared spectroscopy of tissue specific progenitor cell differentiations as a quality control of cell sources for regenerative medicine. In the present study, as a basic study, we examine the adipose differentiation kinetics of preadipose cells (3T3-L1) and the osteoblast differentiation kinetics of mesenchymal stem cells (Kusa-A1) to analyze the infrared absorption spectra.

© 2007 SPIE

PDF Article
More Like This
Regulation of mesenchymal stromal cells differentiation by a blue laser irradiation

Toshihiro Kushibiki and Kunio Awazu
6632_54 European Conference on Biomedical Optics (ECBO) 2007

Advances in FT-IR spectroscopic imaging microscopy of biological tissue using infrared focal-plane array detectors

Linda H. Kidder, Abigail S. Haka, Ira W. Levin, and E. Neil Lewis
TuA2 Biomedical Optical Spectroscopy and Diagnostics (BOSD) 2000

FT-Infrared Spectroscopic Studies of Lymphoma, Lymphoid and Myeloid Leukaemia Cell Lines

Jaspreet Babrah, Keith P. McCarthy, Richard Lush, Adam D. Rye, Conrad Bessant, and Nicholas Stone
6628_28 European Conference on Biomedical Optics (ECBO) 2007


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access Optica Member Subscription

Select as filters

Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved