In vivo multiphoton tomography: a non invasive powerful tool for biochemical investigation of human skin

Ronan Le Harzic; Ronan Le Harzic; Anne Colonna; Rainer Bückle; Alexander Ehlers; Christophe Hadjur; Frederic Leroy; Frederic Flament; Roland Bazin; Bertrand Piot; Iris Riemann; Karsten König

doi:10.1364/ECBO.2007.6630_30

Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2007),
paper 6630_30
•https://doi.org/10.1364/ECBO.2007.6630_30

In vivo multiphoton tomography: a non invasive powerful tool for biochemical investigation of human skin

Ronan Le Harzic, Anne Colonna, Rainer Bückle, Alexander Ehlers, Christophe Hadjur, Frederic Leroy, Frederic Flament, Roland Bazin, Bertrand Piot, Iris Riemann, and Karsten König

European Conference on Biomedical Optics 2007

Munich Germany
17–21 June 2007
ISBN: 9780819467744

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MICROSCOPY IN DERMATOLOGY (MD)

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R. Le Harzic, A. Colonna, R. Bückle, A. Ehlers, C. Hadjur, F. Leroy, F. Flament, R. Bazin, B. Piot, I. Riemann, and K. König, "In vivo multiphoton tomography: a non invasive powerful tool for biochemical investigation of human skin," in Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, A. Periasamy, ed., Vol. 6630 of Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2007), paper 6630_30.

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Abstract

In vivo simultaneous collagen and elastin measurements using the multiphoton tomograph DermaInspect have been performed in skin dermis. We showed it was possible to get simultaneous measurements of autofluorescence (AF) and Second Harmonic Generation (SHG) with a newly developed device using 2 PMTs for time-correlated single photon counting. Unlike elastin (AF), collagen structures are able to generate second harmonics (SHG). Comparing the images and SHG / AF ratios recorded in the depth of the outer and inner sides of the forearm of two European female volunteers (31 and 60 years old, respectively) shows differences in collagen and elastin fibres density. It decreases with depth in the 60 years old volunteer compared to the younger one, and the skin of younger volunteer shows more collagen.

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