Abstract

This paper describes two setups suitable for interference microscopy for high resolution morphological measurements on living cells in culture medium. The first system incorporated a PZT actuator in the reference path of a Mach Zehnder configuration to facilitate digital phase-stepping interferometry. The second system employed two phase-locked acoustooptic modulators to generate a temporal optical carrier to allow a heterodyne approach to phase demodulation. This setup incorporated a digital CMOS camera with full random pixel access which allowed the heterodyne approach to be implemented as a full-field method without any need for electromechanical scanning. The heterodyne approach offers benefits over the phase-stepping method in terms of measurement resolution and speed, typically offering the equivalent of nanometer resolution for cell height measurements with a bandwidth in the order of 200–300 Hz for 1000 pixels. Results for morphological measurements using both systems on red blood cells and keratinocytes are presented.

© 2007 SPIE

PDF Article
More Like This
Non-invasive single cell tomography on skin cells

Nai-Chia Cheng, Chien-Chung Tsai, Tuan-Shu Ho, Ming-Yi Lin, Jeng-Wei Tjiu, and Sheng-Lung Huang
JT2A.7 Bio-Optics: Design and Application (BODA) 2013

Quantitative measurements of dynamic cell morphometry and intracellular integral refractive index with Digital holographic microscopy

Pierre Marquet, Pierre J. Magistretti, Benjamin Rappaz, Tristan Colomb, Jonas Kühn, and Christian Depeursinge
ME24 Biomedical Topical Meeting (BIO) 2006

Techniques and Applications of Digital Holographic Microscopy for Life Cell Imaging

Björn Kemper, Patrik Langehanenberg, Ilona Bredebusch, Jürgen Scbnekenburger, and Gert von Bally
6633_12 European Conference on Biomedical Optics (ECBO) 2007

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription