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Development of microfluidic structures for high throughput flow cytometric characterization of blood cells

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We exploited ultraprecision milling to fabricate three dimensional microfluidic structures incorporating cascaded hydrodynamic focusing. Stable operation when varying the sample flow over three orders of magnitude was demonstrated. Impedance detection or forward light scatter served to detect polystyrene microspheres and to differentiate red blood cells and blood platelets.

© 2007 SPIE

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