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Conferences > ECBO > 2009 > LHP > 7367_10

Live cell imaging with Surface Plasmon-Mediated Fluorescence Microscopy

Karla Balaa, Viviane Devauges, Yannick Goulam, Vincent Studer, Sandrine Lévêque-Fort, and Emmanuel Fort

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Abstract

We present a new imaging technique using surface-plasmon mediated fluorescence microscopy. It uses a similar configuration as standard prismless Total Internal Reflection Fluorescence Microscopy with an additional metallic thin film. In the case of a silver thin film we show that this technique offers many advantages: distance dependence emission filter for improved signal to noise ratio and enhanced molecular detection efficiency. This technique is of particular interest in membrane and adhesion imaging. We present real time images on live cells.

© 2009 OSA/SPIE

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