FRET detection for neurobiological applications using a Total Internal Reflection Fluorescence Lifetime Imaging Microscope

V. Devauges; V. Devauges; V. Devauges; P. Blandin; P. Blandin; P. Blandin; J. C. Cossec; S. Lécart; C. Marquer; M.C. Potier; F. Druon; F. Druon; P. Georges; P. Georges; S. Lévêque-Fort; S. Lévêque-Fort

doi:10.1364/ECBO.2009.7367_11

Advanced Microscopy Techniques
Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2009),
paper 7367_11
•https://doi.org/10.1364/ECBO.2009.7367_11

FRET detection for neurobiological applications using a Total Internal Reflection Fluorescence Lifetime Imaging Microscope

V. Devauges, P. Blandin, J. C. Cossec, S. Lécart, C. Marquer, M.C. Potier, F. Druon, P. Georges, and S. Lévêque-Fort

European Conference on Biomedical Optics 2009

Munich Germany
14–18 June 2009
ISBN: 9780819476432

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V. Devauges, P. Blandin, J. C. Cossec, S. Lécart, C. Marquer, M. C. Potier, F. Druon, P. Georges, and S. Lévêque-Fort, "FRET detection for neurobiological applications using a Total Internal Reflection Fluorescence Lifetime Imaging Microscope," in Advanced Microscopy Techniques, P. Campagnola, E. Stelzer, and G. Von Bally, eds., Vol. 7367 of Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2009), paper 7367_11.

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Abstract

We present the development of a time resolved TIRF microscope illuminated by a supercontinuum laser source. It permits to perform wide-field fluorescence lifetime imaging of neurobiological processes at the plasma membrane with subwavelength axial resolution.

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