Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Study of 3D cell morphology and effect on light scattering distribution

Not Accessible

Your library or personal account may give you access

Abstract

We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.

© 2009 OSA/SPIE

PDF Article
More Like This
Diffraction Imaging Flow Cytometric and 3D Morphological Analysis of Three Cell Lines

Kenneth M. Jacobs, Junhua Ding, Li V. Yang, Carissa L. Reynolds, Andrew E. Ekpenyong, Yuanming Feng, Mary A. Farwell, Jun Q. Lu, and Xin-Hua Hu
BTuD44 Biomedical Optics (BIOMED) 2010

Modeling of light scattering by B-cells using a parallel FDTD method

R. Scott Brock, Xin-Hua Hu, Douglas A. Weidner, Yuanming Feng, and Jun Q. Lu
FTuG48 Frontiers in Optics (FiO) 2004

3D Reconstruction of B-lymphocytes with Confocal Images and Parallel FDTD Simulations of Light Scattering

R. Scott Brock, Xin-Hua Hu, Douglas A. Weidner, Judith R. Mourant, and Jun Q. Lu
MC8 Biomedical Topical Meeting (BIOMED) 2006

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved