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Conferences > ECBO > 2009 > TLCI > 7370_07

Improving FRET Detection in Living Cells

Ching-Wei Chang, Mei Wu, Sofia D. Merajver, and Mary-Ann Mycek

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Abstract

Unambiguous FRET detection in living cells is often challenging. Here we describe how the advantages of fluorescence lifetime sensing with FLIM, fluorophore selection, and critical environmental controls provide better FRET detection.

© 2009 OSA/SPIE

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