Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Pulse Laser Assist optical Tweezers (PLAT) with long-duration pulse laser

Not Accessible

Your library or personal account may give you access


Optical tweezers is a technique to trap and to manipulate micron sized objects under a microscope by radiation pressure force exerted by a laser beam. Optical tweezers has been utilized for single-molecular measurements of force exerted by molecular interactions and for cell palpation. To extend applications of optical tweezers we have developed a novel optical tweezers system combined with a pulse laser. We utilize a pulse laser (Q-switched Nd: YAG laser, wavelength of 1064 nm) to assist manipulations by conventional optical tweezers with a continuous wave (CW) laser. The pulse laser beam is introduced into the same optics for conventional optical tweezers. In principle, instantaneous radiation force is proportional to instantaneous power of laser beam. As a result, pulse laser beam generates strong instantaneous force on an object to be manipulated. If the radiation force becomes strong enough to get over an obstacle structure and/or to be released from adhesion, the object will be free from these difficulties. We investigate the effect of pulse laser assistance with changing pulse duration of the laser. We report optimum pulse duration of 100 ns to 200 ns deduced from motion analysis of a particle in a beam spot. Our goal is to realize in-vivo manipulation and operation of a cell. For this purpose we need to reduce light energy of pulse laser beam and to avoid laser induced breakdown caused by strong light field. So we have developed a pulse laser with 160-ns pulse duration and have confirmed that availability on manipulation of living cells.

© 2011 OSA/SPIE

PDF Article
More Like This
Red blood cell micromanipulation with elliptical laser beam profile optical tweezers in different osmolarity conditions

E. Spyratou, M. Makropoulou, and A. A. Serafetinides
80920T European Conference on Biomedical Optics (ECBO) 2011

Investigation of mechanical property of a cell by optical tweezers

T. Sugiura
880105 European Conference on Biomedical Optics (ECBO) 2013

Enhancement of optical gradient force employed in optical tweezers using a pulsed laser diode

Takamasa Suzuki, Takatsugu Maeda, Osami Sasaki, and Samuel Choi
OTMD4p Optical Trapping Applications (OMA) 2011

Select as filters

Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.