Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Vertical Differential Interference Contrast using SLMs

Not Accessible

Your library or personal account may give you access

Abstract

In microscopy, several different phase contrast methods (e.g. Zernike contrast, differential interference contrast) have been developed to image phase objects. For these methods specialized microscopic equipment (modified microscope objectives, filters, etc.) is needed. The static elements within such microscopes are a trade-off, because phase contrast imaging depends strongly on the object and the information to be visualized. We show results of an implementation of many different phase contrast methods using a high resolution phase-only spatial light modulator (SLM) in the pupil plane. All implemented methods are realized by software. Therefore, it is not only feasible to change the different phase contrast methods in real time, but it is also possible to optimize the parameters. Images obtained from different settings are combined digitally to improve the final image quality. Furthermore, completely new phase contrast filters can be tested easily because the phase of each pixel can be changed arbitrarily. We use this method to implement a new phase contrast filter that is obtained by combining a focused and a defocused point spread function. We will present theoretical as well as experimental results of this vertical differential interference contrast filter.

© 2011 OSA/SPIE

PDF Article
More Like This
Quantitative Differential Interference Contrast Microscopy

Michael Shribak
MTu4A.5 Microscopy Histopathology and Analytics (Microscopy) 2018

Spectral-domain Differential Interference Contrast Microscopy

Yizheng Zhu, Natan T. Shaked, Lisa L. Satterwhite, and Adam Wax
NTuB4 Novel Techniques in Microscopy (NTM) 2011

Dynamic Differential Image Contrast by Digital Holography for imaging and Quantitative Phase Microscopy

L. Miccio, A. Finizio, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro
80860D European Conference on Biomedical Optics (ECBO) 2011

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved