Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Infrared spectroscopy to estimate the gross biochemistry associated with different colorectal pathologies

Not Accessible

Your library or personal account may give you access

Abstract

Histopathology provides the gold standard assessment of colonoscopic biopsies. Infrared spectroscopy can potentially map biochemical changes across a tissue section identifying disease. The purpose of this study was to determine if infrared spectroscopy could classify different colorectal pathologies and to investigate biochemical composition. Colonoscopic tissue biopsies were snap frozen at colonoscopy. 10 micron thick sections were mounted on CaF2 slides. 3-D spectral datasets (2 spatial dimensions and one spectral) were measured from thawed specimens using a Perkin Elmer infrared imaging system in transmission mode. Contiguous tissue sections stained with H&E were reviewed by a specialist gastrointestinal pathologist for comparison. Tissue spectra from epithelial tissues were classified using principal components fed linear discriminant analysis with leave one out cross validation. Reference spectra from purchased biochemicals (Sigma-Aldrich) were measured. Ordinary least squares analysis estimated the relative biochemical signal contribution from epithelial regions. Spectra from tissue epithelia measured from normal tissue, hyperplastic polyps, adenomatous polyps, cancer and ulcerative colitis samples were classified with accuracies in excess of 90%. Ordinary least squares analysis demonstrated a higher DNA to cytoplasm ratio in cancer compared to normal tissue. FTIR spectra from epithelia can be used to classify colorectal pathologies with high accuracy. Ordinary least squares analysis shows promise for extraction of useful biochemical information. These techniques could aid the histopathologist and ultimately lead to automated histopathological processing.

© 2011 OSA/SPIE

PDF Article
More Like This
Application of laser induced autofluorescence spectra detection in human colorectal cancer screening

Sheng Fu, Teck Chee Chia, Leong Chuan Kwek, Cheong Hoong Diong, Choong Leong Tang, Francis Seow Choen, and S.M. Krishnan
5141_298 European Conference on Biomedical Optics (ECBO) 2003

Correlative Studies of Autofluorescence, Immuno/histopathology and Infrastructures of Ex Vivo Tissues and Primary Cultured Epithelial Cells from Human Normal, Preneoplastic and Neoplastic Colorectal Mucosa.

Ralph S. DaCosta, Helene Andersson, Brian C. Wilson, Maria Cirocco, Shirley Hassaram, and Norman E. Marcon
BTuC4 Biomedical Topical Meeting (BIO) 1999

Study on image feature extraction and classification for human colorectal cancer using optical coherence tomography

Shu-Wei Huang, Shan-Yi Yang, Wei-Cheng Huang, Han-Mo Chiu, and Chih-Wei Lu
80911Y European Conference on Biomedical Optics (ECBO) 2011

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved