Abstract
Based on laser technology it is possible to visualize nanoparticles (NP) as light scattering objects by means of a conventional light microscope. Image series of visualized NPs were used to calculate characteristic parameters such as particle size and diffusion coefficient. Here progressive agglomeration of NP upon changes of ionic strength could be followed by repeated observation over minutes. While these analyses were based on dynamic properties of NPs, single particle paths were used to analyse particle agglomeration in more detail using image analysis methods. Together these techniques may help to better describe the behaviour of nanoparticles when used for cell culture experiments.
© 2011 OSA/SPIE
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