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Hyperspectral Fluorescence Tomography Of Quantum Dots Using The Simplified Spherical Harmonics Equations

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Abstract

Unlike high-energy gamma radiation used in nuclear imaging, light in the visible and near-infrared from a fluorescence source is strongly scattered in biological tissue yielding images of diffuse light intensity distributions on the tissue surface. These images contain little information about the actual depth and strength of the source of fluorescence inside tissue. As illustrated in Figure 1, similar surface images may be obtained for a weak fluorescence source (small power density) near the surface and a strong fluorescence source (large power density) deep inside tissue. Therefore, tomographic imaging is needed in order to retrieve the three-dimensional (3D) information about the “true” fluorescence source location and power density.

© 2011 OSA/SPIE

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