Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Structural analysis of artificial skin equivalents

Not Accessible

Your library or personal account may give you access

Abstract

Artificial skin equivalents ASEs based on primary fibroblasts and keratinocytes show a high batch variance in their structural and morphological characteristics. Due to biological fluctuations and variable donor age, the growth processes of 3D tissue structure show a non constant quality. Since theses ASEs are used as testing system for chemicals, pharmaceuticals or cosmetics it is of major interest to know detailed and significant characteristics about each individual ASE. Until now, the microscopic analysis process is based on the destructive preparation of histologies allowing only the characterization on a random basis. In this study we present analytical methods to characterise each individual ASE by Optical Coherence Tomography OCT in combination with image processing tools. Therefore, we developed a fully automated OCT device, that performs automatic measurements of microtiter plates MTPs holing the ASEs in a sterile environment. We developed image processing algorithms to characterize the surface structure which may function as an indicator for defects in the epidermal stratum corneum. Further, we analysed the tomographic morphological structure of the ASEs. The results show, that variances in the growth state as well different collagen formation is detectable. In combination with dynamic threshold levels, we found, that OCT is a well suited technology for automatically characterizing artificial skin equivalents and may partly substitute the preparation of histologies.

© 2011 OSA/SPIE

PDF Article
More Like This
Non-Destructive Detection of Defects in Artificial Skin Tissue by Optical Coherence Tomography

R. Schmitt, U. Marx, A. Heymer, and M. Kaufmann
7372_2F European Conference on Biomedical Optics (ECBO) 2009

Simultaneous Optical Coherence and Multiphoton Microscopy of Skin-Equivalent Tissue Models

Jennifer K Barton, Shuo Tang, Ryan Lim, and Bruce J. Tromberg
6627_26 European Conference on Biomedical Optics (ECBO) 2007

Metabolic and morphological evaluation of an artificial full-thickness skin model using a multimodal photonic system

Arooj Khalid, Viktor Dremin, Ayman El-Tamer, Maria Surnina, Celine Lancelot, Edik Rafailov, and Sergei Sokolovski
126290T European Conference on Biomedical Optics (ECBO) 2023

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved