Detection of bacterial infection with a fiber optic microendoscope

Nooman Mufti; Ying Kong; Jeffrey D. Cirillo; Kristen C. Maitland

doi:10.1364/ECBO.2011.80920A

Medical Laser Applications and Laser-Tissue Interactions V
Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2011),
paper 80920A
•https://doi.org/10.1364/ECBO.2011.80920A

Detection of bacterial infection with a fiber optic microendoscope

Nooman Mufti, Ying Kong, Jeffrey D. Cirillo, and Kristen C. Maitland

European Conference on Biomedical Optics 2011

Munich Germany
22–26 May 2011
ISBN: 9780819486899

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N. Mufti, Y. Kong, J. D. Cirillo, and K. C. Maitland, "Detection of bacterial infection with a fiber optic microendoscope," in Medical Laser Applications and Laser-Tissue Interactions V, R. Sroka and L. Lilge, eds., Vol. 8092 of Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2011), paper 80920A.

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Abstract

We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 ȝm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 10⁶ to 10¹ CFU and intra-tracheal inocula of 10⁸ to 10⁶ CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 10⁴ CFU for skin samples and 10⁷ CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 10⁶ CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples.

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