Abstract
We developed a method to determine the optical properties of biological tissue from the results of integrating sphere measurements. Our Monte Carlo model developed for this purpose considered the geometry of the investigated sample as well as the features of the integrating sphere setup. To improve the accuracy of our results, we incorporated the wavelength dependence of the anisotropy factor of the investigated tissue into the Monte Carlo model. To determine the anisotropy factor, we performed goniometric measurements on six porcine dura mater tissue samples and quantified the phase function at a wavelength of 650 nm. The averaged anisotropy factor was taken into account in the Monte Carlo simulations. The result of these simulations were combined in a table lookup. We used this table lookup to interpret the results from the integrating sphere measurements. We present as a result of our measurements the absorption coefficient and the reduced scattering coefficient of porcine dura mater tissue in the wavelength range of 450 to 650 nm.
© 2011 OSA/SPIE
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