Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

In vitro analysis of low - level laser irradiation on human osteoblast-like cells proliferation

Not Accessible

Your library or personal account may give you access


The objective of this study was to examine the in vitro effect of a single or a multiple doses of low-level laser irradiation (LLLI) on proliferation of the human osteosarcoma cell line, SAOS-2. SAOS-2 cells were divided in five groups and exposed to LLLI (659 nm diode laser; 11 mW power output): group I as a control (dark), group II exposed to a single laser dose of 1 J/cm, group III irradiated with a single dose of 3 J/cm, and group IV and V exposed for three consecutive days to 1 or 3 J/cm, respectively. Cellular proliferation was assessed daily up to 7 days of culturing. The obtained results showed an increase in proliferative capacity of SAOS-2 cells during the first 96 h of culturing time in once-irradiated cells, as compared to control cells. Furthermore, a significantly higher proliferation in the group IV and V was detected if compared to a single dose or to control group after 96 h and 7 days. In conclusion, the effect of the single dose on cell proliferation was transitory and repeated irradiations were necessary to observe a strong enhancement of SAOS-2 growth. As a future perspective, we would like to determine the potential of LLLI as a new approach for promoting bone regeneration onto biomaterials.

© 2011 OSA/SPIE

PDF Article
More Like This
Efficacy of a single high dose versus multiple low doses of LLLT on wounded skin fibroblasts

Denise H. Hawkins and Heidi Abrahamse
6632_46 European Conference on Biomedical Optics (ECBO) 2007

Biochemical and topological analysis of bovine sperm cells induced by low power laser irradiation

T. R. Dreyer, A. F. P. Siqueira, T. D. Magrini, P. A. Fiorito, M. E. O. A. Assumpção, M. Nichi, H. S. Martinho, and M. P. Milazzotto
80920V European Conference on Biomedical Optics (ECBO) 2011

Evaluation of LED therapy at 945 nm on bone repair by micro x-ray fluorescence spectroscopy and scanning electron microscopy

Alexandre G. Diamantino, Renata A. Nicolau, Marco A. de Oliveira, and Ana Maria E. Santo
80921S European Conference on Biomedical Optics (ECBO) 2011

Select as filters

Select Topics Cancel
© Copyright 2023 | Optica Publishing Group. All Rights Reserved