Quantification of Inflammation in Colonic Tissue Sections and Wound Healing In-Vitro with Digital Holographic Microscopy

Dominik Bettenworth; Philipp Lenz; Philipp Krausewitz; Markus Brückner; Steffi Ketelhut; Gert von Bally; Dirk Domagk; Björn Kemper

Advanced Microscopy Techniques III
SPIE Proceedings (Optica Publishing Group, 2013),
paper 879702

Quantification of Inflammation in Colonic Tissue Sections and Wound Healing In-Vitro with Digital Holographic Microscopy

Dominik Bettenworth, Philipp Lenz, Philipp Krausewitz, Markus Brückner, Steffi Ketelhut, Gert von Bally, Dirk Domagk, and Björn Kemper

European Conference on Biomedical Optics 2013

Munich Germany
12–16 May 2013
ISBN: 9780819496461

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Biomedical Applications I (BAI)

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D. Bettenworth, P. Lenz, P. Krausewitz, M. Brückner, S. Ketelhut, G. von Bally, D. Domagk, and B. Kemper, "Quantification of Inflammation in Colonic Tissue Sections and Wound Healing In-Vitro with Digital Holographic Microscopy," in Advanced Microscopy Techniques III, E. Beaurepaire and P. So, eds., Vol. 8797 of SPIE Proceedings (Optica Publishing Group, 2013), paper 879702.

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Abstract

We show that the tissue refractive index, obtained from quantitative digital holographic microscopy (DHM) phase contrast images of unstained histological colonic sections, is directly related to the degree of inflammation in experimental colitis. In addition, it is demonstrated that quantitative DHM phase contrast is capable to quantify in-vitro wound healing assays.

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