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Optica Publishing Group
  • Advanced Microscopy Techniques III
  • SPIE Proceedings (Optica Publishing Group, 2013),
  • paper 87970A

High Resolution Quantitative Phase Imaging in Digital Holographic Microscopy by Modulated Object Illumination with an Electrically Focusable Lens

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Abstract

In digital holographic microscopy (DHM), scattering patterns that are induced by coherent laser light affect the resolution for the detection of optical path length changes. We present a simple and efficient approach for the reduction of coherent disturbances in quantitative phase imaging in self-interference DHM that is based on amplitude and phase modulation of the sample illumination. The performance of the method for quantitative phase imaging is characterized and the application for quantitative analysis of living cells is illustrated.

© 2013 SPIE

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