In vivo two-photon imaging of climbing fibers plasticity after laser axotomy

A. L. Allegra Mascaro; P. Cesare; P. Cesare; L. Sacconi; L. Sacconi; G. Grasselli; G. Grasselli; G. Mandolesi; B. Maco; G. W. Knott; V. De Paola; P. Strata; P. Strata; F.S. Pavone; F.S. Pavone; F.S. Pavone; F.S. Pavone

Neurophotonics
SPIE Proceedings (Optica Publishing Group, 2013),
paper 880407

In vivo two-photon imaging of climbing fibers plasticity after laser axotomy

A. L. Allegra Mascaro, P. Cesare, L. Sacconi, G. Grasselli, G. Mandolesi, B. Maco, G. W. Knott, V. De Paola, P. Strata, and F.S. Pavone

European Conference on Biomedical Optics 2013

Munich Germany
12–16 May 2013
ISBN: 9780819498120

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A. L. Allegra Mascaro, P. Cesare, L. Sacconi, G. Grasselli, G. Mandolesi, B. Maco, G. W. Knott, V. De Paola, P. Strata, and F. S. Pavone, "In vivo two-photon imaging of climbing fibers plasticity after laser axotomy," in Neurophotonics, F. Pavone, E. Hillman, V. Daria, and S. Charpak, eds., Vol. 8804 of SPIE Proceedings (Optica Publishing Group, 2013), paper 880407.

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Abstract

In the adult nervous system, different neuronal classes show different regenerative behavior. Although previous studies demonstrated that olivocerebellar fibers are capable of axonal regeneration in a suitable environment as a response to injury, we have hitherto no details about the real dynamics of fiber regeneration. We set up a model of singularly axotomized climbing fibers (CF) to investigate their reparative properties in the adult central nervous system (CNS) in vivo. Here we describe the approach followed to characterize the reactive plasticity after injury.

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