Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Comparison of the polarization properties in the retinas of different rodents using high resolution polarization sensitive OCT

Not Accessible

Your library or personal account may give you access


Animal models play an important role for understanding the pathophysiology of glaucoma and age-related macular degeneration. With these models, longitudinal studies can be performed and therefore there is need for non-invasive evaluation of disease progress. For that purpose optical coherence tomography (OCT) can be used. Since tissues with different polarization properties are important in these diseases, polarization sensitive OCT (PS-OCT) could be a valuable tool in preclinical research. In this work a high resolution PS-OCT (HR-PS-OCT) system was used in-vivo for rodent retinal imaging. A superluminescent diode with a bandwidth of 100 nm was used as a light source that yielded an axial resolution of 5.1 μm in air (3.8 μm in tissue). The A-scan rate was 83 kHz, a whole 3D dataset was acquired in a few seconds (1536×1024×200 pixels in 3.5 s) which reduced motion artifacts. Rats (Sprague-Dawley, Long-Evans and Brown Norway) as well as mice (C57BL/6) were imaged. High resolution reflectivity images showed all retinal layers in all animals. From acquired data also phase retardation, fast axis orientation and degree of polarization uniformity (DOPU) images were calculated. On phase retardation images sclera was identified as birefringent and retinal pigment epithelium (RPE) and choroid as depolarizing tissues. Our results demonstrate the suitability of the system for high speed/resolution imaging in follow up studies on rodents.

© 2015 SPIE

PDF Article
More Like This
In Vivo Imaging of Retinal and Choroidal Vasculature in the Rodent Eye using Optical Coherence Tomography

Marco Augustin, Stanislava Fialová, Roberto Plasenzotti, Michael Pircher, Christoph K. Hitzenberger, and Bernhard Baumann
95410O European Conference on Biomedical Optics (ECBO) 2015

Imaging of the Human Retina by Polarization Sensitive and Cellular Resolution OCT

Christoph K. Hitzenberger
FTuQ6 Frontiers in Optics (FiO) 2011

High-speed polarization-sensitive optical coherence tomography (PS-OCT) at 1060 nm

Teresa Torzicky, Michael Pircher, Sebastian Marschall, Marco Bonesi, Stefan Zotter, Erich Götzinger, Thomas Klein, Wolfgang Wieser, Benjamin Biedermann, Robert Huber, Peter Andersen, and Christoph K. Hitzenberger
BTu2B.5 Biomedical Optics (BIOMED) 2012


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access Optica Member Subscription

Select as filters

Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved